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Updated: Dec 16, 2025

An Innovative Method for Exosome Quantification and Size Measurement

An Innovative Method for Exosome Quantification and Size Measurement

Published on: January 17, 2015

Exosome Purification and Analysis Using a Facile Microfluidic Hydrodynamic Trapping Device.

Mahnoush Tayebi¹, Yinning Zhou¹, Pallavi Tripathi¹

¹Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore.

Analytical Chemistry

|July 3, 2020

Summary

This summary is machine-generated.

This study presents a novel microfluidic device for isolating and quantifying exosomes (extracellular vesicles). The method uses affinity-based microbeads and hydrodynamic trapping for precise exosome analysis and molecular profiling.

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Last Updated: Dec 16, 2025

An Innovative Method for Exosome Quantification and Size Measurement

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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Area of Science:

Biotechnology
Nanotechnology
Analytical Chemistry

Background:

Exosomes (extracellular vesicles) are vital biomarkers for disease diagnosis and prognosis.
Efficient isolation of exosomes from complex biological fluids is crucial for clinical applications.
Current exosome purification methods require improvement for precision and reliability.

Purpose of the Study:

To develop an efficient and reliable method for isolating and quantifying exosomes.
To enable precise characterization of exosome subpopulations.
To facilitate downstream molecular analysis of captured exosomes.

Main Methods:

Functionalization of microbeads with antibodies for exosome capture via antigen-antibody affinity.
Development of a microfluidic device with passive hydrodynamic trapping for single-bead isolation.
Massively multiplexed fluorescence detection for exosome quantification.
On-chip elution and lysis for protein and RNA analysis.

Main Results:

Successful isolation and enrichment of exosomes using functionalized microbeads.
Efficient trapping of single microbeads within the microfluidic device.
Accurate exosome quantification through fluorescence detection.
Demonstrated capability for downstream molecular analysis (Western blot, qPCR).

Conclusions:

The developed microfluidic device offers a rapid and straightforward method for exosome capturing and quantification.
This technique enables precise analysis of extracellular vesicles for diverse biological studies.
The approach holds potential for advancing diagnostic and prognostic applications of exosomes.