Related Experiment Video
Updated: Aug 8, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of
Stefan Monecke1,2,3,4, Elisabeth König4,5, Megan R Earls4,6
1Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
Abstract:
We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.
More Related Videos
09:03Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
09:07Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
Published on: May 24, 2024
Related Concept Videos
Rapid Identification of Pathogens
Mechanism of Antibiotic Resistance in MRSA
Clinical Significance of Antibiotic Resistance