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Related Concept Videos

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Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
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Related Experiment Video

Updated: Dec 16, 2025

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

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Metabolic changes in mouse sperm during capacitation†.

Melanie Balbach1, Maria Gracia Gervasi2, David Martin Hidalgo2

  • 1Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA.

Biology of Reproduction
|July 3, 2020
PubMed
Summary
This summary is machine-generated.

Capacitating mouse sperm increase glucose uptake and energy production through glycolysis and oxidative phosphorylation. This metabolic shift is crucial for sperm motility and fertilization readiness.

Keywords:
capacitationenergy productionglucose uptakeglycolysisoxidative phosphorylation

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Area of Science:

  • Reproductive biology
  • Sperm metabolism
  • Cellular energetics

Background:

  • Mammalian sperm are stored dormant in the epididymis.
  • Upon ejaculation, sperm require immediate energy for motility and capacitation.
  • The metabolic adaptations of mouse sperm during capacitation are not fully understood.

Purpose of the Study:

  • To investigate metabolic changes in mouse sperm during capacitation.
  • To identify key metabolic pathways supporting sperm function post-ejaculation.
  • To establish a link between glycolysis and oxidative phosphorylation in sperm.

Main Methods:

  • Measurement of glucose uptake in capacitating mouse sperm.
  • Utilizing extracellular flux analysis to assess cellular respiration.
  • Investigating the interplay between glycolysis and oxidative phosphorylation.

Main Results:

  • Capacitating mouse sperm exhibit enhanced glucose uptake, serving as a functional marker.
  • Both glycolysis and oxidative phosphorylation rates increase during sperm capacitation.
  • Oxidative phosphorylation is shown to be dependent on glycolysis in mouse sperm.

Conclusions:

  • Mouse sperm enhance glucose uptake during capacitation to meet increased energy demands.
  • Sperm capacitation involves coordinated increases in glycolysis and oxidative phosphorylation.
  • A functional link exists between glycolysis and oxidative phosphorylation in mammalian sperm metabolism.