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Decrease of intracellular ROS by arbutin is associated with apoptosis induction and downregulation of IL-1β and TNF-α

Hajar Safari1, Ebrahim Zabihi2, Mahdi Pouramir2

  • 1Student Research Committee, Babol University of Medical Sciences, Babol, Iran.

Journal of Food Biochemistry
|July 3, 2020
PubMed

Insights

Arbutin, an antioxidant, reduced reactive oxygen species (ROS) and inflammation in prostate cancer cells. This study suggests arbutin

Area of Science:

  • Oncology
  • Biochemistry
  • Pharmacology

Background:

  • Prostate cancer (PCa) is associated with increased reactive oxygen species (ROS) and inflammation.
  • Arbutin, a natural antioxidant, has demonstrated anti-inflammatory and anti-melanoma properties.
  • Investigating arbutin's effects on PCa provides potential complementary therapeutic strategies.

Purpose of the Study:

  • To investigate the molecular mechanisms of arbutin's antioxidant effects on the LNCap prostate cancer cell line.
  • To evaluate arbutin's impact on ROS levels, cell death, and inflammatory gene expression in PCa cells.

Main Methods:

  • In vitro study using the LNCap prostate cancer cell line.
  • Measurement of intracellular reactive oxygen species (ROS) levels.
  • Assessment of cell death profiles (apoptosis and necrosis) and gene/protein expression of inflammatory markers (IL-1β, TNF-α).

Main Results:

  • Arbutin significantly decreased intracellular ROS levels in a dose-dependent manner.
  • Arbutin (1,000 μM) induced apoptosis, whereas tert-butyl hydroperoxide (tBHP) induced necrosis.
  • Arbutin reduced the expression of pro-inflammatory genes (IL-1β, TNF-α) and IL-1β protein levels.

Conclusions:

  • Arbutin exhibits antioxidant and anti-inflammatory effects in prostate cancer cells by reducing ROS and pro-inflammatory cytokines.
  • Arbutin promotes apoptosis in PCa cells, suggesting a potential role in cancer therapy.
  • These findings highlight arbutin's potential as a complementary medicine approach for prostate cancer treatment.
Keywords:
IL-1βROSTNF-αantioxidantarbutinprostate cancer

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