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In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
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Traceable Peptidic Ligands that Target Ghrelin Receptors

Mengjie Liu1, John Wade1, Mohammed Akhter Hossain1

  • 1Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC 3010, Australia.

Current Protein & Peptide Science
|July 3, 2020
PubMed

Insights

This review covers 12 traceable ligands for visualizing ghrelin signaling. These radio- and fluorescently-labeled peptides aid in understanding ghrelin

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Background:

  • Ghrelin, a 28-amino acid peptide hormone, plays a key role in numerous physiological and pathophysiological processes.
  • Understanding the ghrelin signaling pathway requires precise visualization of ghrelin and its receptor.
  • Traceable ligands are essential tools for studying the localization, function, and expression of ghrelin's pathway.

Purpose of the Study:

  • To review representative radio- and fluorescently-labeled peptide-based ligands for ghrelin imaging.
  • To focus on the structural characteristics and pharmacological properties of these imaging agents.
  • To highlight their applications in in vitro and in vivo studies and further biochemical research.

Main Methods:

  • Review of 12 distinct peptide-based ligands.
  • Analysis of radio- and fluorescent labeling techniques.
  • Evaluation of in vitro and in vivo imaging applications.

Main Results:

  • Compilation of 12 representative traceable ligands for ghrelin visualization.
  • Detailed examination of ligand structures and their pharmacological profiles.
  • Summary of current applications in imaging and biochemical studies.

Conclusions:

  • Traceable ligands are vital for advancing ghrelin research.
  • The reviewed ligands offer diverse options for studying ghrelin signaling pathways.
  • These tools facilitate deeper biochemical insights and therapeutic target identification.
Keywords:
fluorescenceghrelinghrelin receptorligandpeptideradiolabeling

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