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ROR2 knockdown suppresses breast cancer growth through PI3K/ATK signaling

Muhong Guo1, Ge Ma2, Xiaolan Zhang3

  • 1Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

Aging
|July 3, 2020
PubMed

Insights

Receptor tyrosine kinase-like orphan receptor 2 (ROR2) drives breast cancer progression by promoting cell proliferation and reducing survival. Targeting ROR2 may offer a new therapeutic strategy for breast cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is implicated in various cancers.
  • Its role in breast cancer (BC) progression requires further elucidation.

Purpose of the Study:

  • To investigate the clinical significance of ROR2 in breast cancer (BC) progression.
  • To determine the functional role of ROR2 in BC cell proliferation, growth, and tumorigenesis.

Main Methods:

  • Analysis of ROR2 mRNA levels in BC tissues and adjacent non-tumor tissues.
  • Kaplan-Meier disease-free survival (DFS) analysis.
  • In vitro cell proliferation and apoptosis assays.
  • In vivo mouse BC xenograft models.
  • Investigation of the PI3K/AKT signaling pathway.

Main Results:

  • ROR2 expression was significantly elevated in BC tissues and correlated with tumor diameter.
  • Higher ROR2 expression was associated with lower DFS in BC patients.
  • ROR2 knockdown suppressed BC cell proliferation and induced apoptosis, while ROR2 overexpression had opposite effects.
  • ROR2 suppression reduced tumor growth in vivo.
  • ROR2 promotes BC cell proliferation by activating the PI3K/AKT pathway.

Conclusions:

  • ROR2 functions as an oncogenic gene in breast cancer.
  • The ROR2/PI3K/AKT regulatory network is crucial for BC progression.
  • ROR2 represents a potential therapeutic target for breast cancer treatment.
Keywords:
PI3K/AKT signalingROR2apoptosisbreast cancer cellproliferation

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