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Updated: Dec 16, 2025

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Precipitation-based colorimetric multiplex immunoassay in hydrogel particles.

Yoon Ho Roh1, Hyun Jee Lee, Ju Yeon Kim

  • 1Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea. bong98@korea.ac.kr.

Lab on a Chip
|July 3, 2020
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Summary

This study introduces a novel colorimetric multiplex immunoassay using hydrogel microparticles for sensitive, portable detection of multiple analytes. This low-cost diagnostic tool offers high sensitivity and dynamic range, outperforming traditional lab-based methods.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Diagnostics

Background:

  • Current diagnostic technologies lack simultaneous multi-analyte detection with portability and sensitivity.
  • Colorimetric assays are simple but not multiplexible.
  • Particle-based assays are multiplexible but require lab equipment.

Purpose of the Study:

  • To develop a portable, low-cost colorimetric multiplex immunoassay.
  • To combine the benefits of colorimetric and particle-based assays.
  • To enable sensitive detection of multiple biomarkers using hydrogel microparticles.

Main Methods:

  • Developed a colorimetric multiplex immunoassay using hydrogel microparticles.
  • Utilized local precipitation and amplification of insoluble color dyes within hydrogel networks.
  • Employed enzymatic accumulation of color dyes and characterized color development kinetics.
  • Analyzed hydrogel microparticles using low-cost portable devices via visible color signals.

Main Results:

  • Achieved high sensitivity and dynamic range comparable to lab-based enzyme-linked immunosorbent assay (ELISA).
  • Successfully detected three preeclampsia biomarkers.
  • Validated the assay using clinical plasma samples.

Conclusions:

  • The hydrogel microparticle-based colorimetric multiplex immunoassay offers a sensitive and portable diagnostic solution.
  • This approach overcomes limitations of existing diagnostic methods.
  • The technology has potential for clinical diagnostics, demonstrated by preeclampsia biomarker detection.