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Profound suppression of lymphocyte function in early biliary obstruction
T D Feduccia1, C E Scott-Conner, J B Grogan
1Department of Surgery, University of Mississippi School of Medicine, Jackson 39216-4505.
The American Journal of the Medical Sciences
|July 1, 1988
Summary
Common bile duct ligation in rats suppresses immune cell responses to mitogens like concanavalin A and phytohemagglutinin. This immune suppression in spleen and lymph nodes occurs early after bile duct ligation and persists.
Area of Science:
- Immunology
- Gastroenterology
- Cell Biology
Background:
- Bile duct ligation (CBD) in rats leads to jaundice and can impact immune function.
- Understanding the effects of cholestasis on immune cell blastogenesis is crucial for managing related complications.
Purpose of the Study:
- To investigate the impact of common bile duct ligation (CBD) on the blastogenic response of spleen and lymph node cells to mitogens.
- To determine the time course and nature of immune cell alterations following CBD in Lewis rats.
Main Methods:
- Lewis rats underwent either common bile duct ligation (CBD) or sham celiotomy (SC).
- Spleen and lymph node cells were isolated at various time points (1-14 days post-procedure).
- Cells were stimulated with mitogens: concanavalin A, phytohemagglutinin, and lipopolysaccharide. Blastogenic responses were measured.
Main Results:
- Splenocytes showed suppressed responses to concanavalin A and phytohemagglutinin as early as 3 days after CBD, persisting throughout the study.
- Lymph node cells exhibited suppressed responses to concanavalin A and phytohemagglutinin by day 4 of jaundice.
- No significant alteration was observed in the splenocyte response to lipopolysaccharide.
- No correlation was found between the observed immune suppression and serum bilirubin levels.
Conclusions:
- Common bile duct ligation induces a significant and persistent suppression of T-cell mediated immune responses in spleen and lymph node cells of Lewis rats.
- The suppression of blastogenic responses is specific to certain mitogens and is not directly correlated with the level of hyperbilirubinemia.