Related Experiment Video
Updated: Dec 16, 2025
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
35.2K
A Tale of Two Moieties: Rapidly Evolving CRISPR/Cas-Based Genome Editing.
1CAS Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Trends in Biochemical Sciences
|July 4, 2020
Summary
Genome editing uses locator and effector moieties for precise genetic changes. CRISPR/Cas systems, including base and prime editing, offer versatile tools, but managing off-target effects remains crucial for future applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Genome editing relies on distinct locator and effector moieties for targeted genetic modifications.
- CRISPR/Cas technology has enabled a diverse range of precise genome editing tools for various applications.
Purpose of the Study:
- To review genome editing systems focusing on locator and effector moieties.
- To highlight advancements in CRISPR-conjugated base and prime editing technologies.
- To discuss off-target effects and mitigation strategies in CRISPR systems.
Main Methods:
- Literature review of genome editing technologies.
- Analysis of CRISPR/Cas system components (locator and effector moieties).
- Examination of base editing and prime editing mechanisms and off-target effects.
Main Results:
- CRISPR/Cas systems provide modularity for target binding and genetic engineering.
- Base editing and prime editing represent significant advances in CRISPR-conjugated systems.
- Distinct off-target mutation mechanisms are identified in CRISPR-conjugated systems.
Conclusions:
- Genome editing systems, particularly CRISPR-based ones, are continuously evolving.
- Understanding and minimizing off-target effects are critical for the safe and effective application of genome editing technologies.
- Future research should focus on developing strategies to reduce off-target mutations.
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