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Updated: Dec 16, 2025
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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Published on: November 16, 2018
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Transcriptome analysis of basic fibroblast growth factor treated stem cells isolated from human exfoliated deciduous
Nunthawan Nowwarote1, Jeeranan Manokawinchoke1,2, Kiattipan Kanjana1
1Center of Excellence for Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330 Thailand.
Heliyon
|July 4, 2020
Summary
Basic fibroblast growth factor (bFGF) promotes stem cell proliferation by regulating cell cycle progression in human exfoliated deciduous teeth derived stem cells. This finding supports bFGF
Area of Science:
- Stem cell biology
- Regenerative medicine
- Molecular biology
Background:
- Basic fibroblast growth factor (bFGF) is crucial for cell proliferation, migration, and differentiation.
- Stem cells from human exfoliated deciduous teeth (SHEDs) are a promising source for regenerative therapies.
Purpose of the Study:
- To identify bFGF target genes in SHEDs.
- To investigate the effect of bFGF on SHED proliferation and cell cycle.
Main Methods:
- SHEDs were isolated and characterized for stem cell markers and differentiation potential.
- RNA sequencing was employed to analyze the transcriptome of bFGF-treated SHEDs.
- Real-time PCR, immunofluorescence, and flow cytometry were used to validate gene and protein expression and cell cycle distribution.
Main Results:
- bFGF upregulated cell cycle-related genes (e.g., MKI67) and downregulated extracellular matrix genes.
- bFGF treatment increased the G2/M phase population and enhanced colony formation in SHEDs.
- FGFR and PI3K inhibitors attenuated the bFGF-induced increase in colony formation.
Conclusions:
- bFGF effectively controls cell cycle progression in SHEDs.
- bFGF can be utilized to amplify SHED numbers for regenerative applications.
