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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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SDS-PAGE01:27

SDS-PAGE

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Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
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Electrophoresis: Overview01:20

Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.3K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

889
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
889
Southern Blot02:57

Southern Blot

22.0K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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Related Experiment Video

Updated: Dec 16, 2025

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

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Carbohydrate Gel Electrophoresis.

Florence Goubet1, Paul Dupree2, Katja Salomon Johansen3

  • 1BASF Belgium Coordination Center Comm.V., Innovation Center Gent, Ghent, Belgium. Florence.Goubet@basf.com.

Methods in Molecular Biology (Clifton, N.J.)
|July 4, 2020
PubMed
Summary

Carbohydrate gel electrophoresis (PACE) analyzes polysaccharides by labeling sugar ends with a fluorophore. This sensitive method aids in studying polysaccharide structure, quantity, and enzyme specificity.

Keywords:
EnzymeHemicelluloseMonosaccharideOligosaccharidePectinPolysaccharide

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Related Experiment Videos

Last Updated: Dec 16, 2025

Agarose Gel Electrophoresis for the Separation of DNA Fragments
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Agarose Gel Electrophoresis for the Separation of DNA Fragments

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Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
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Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

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Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Glycobiology

Background:

  • Polysaccharide analysis is crucial for understanding biological processes.
  • Existing methods may lack sensitivity or simplicity for certain applications.

Purpose of the Study:

  • To introduce and highlight the utility of Polysaccharide Analysis by Chemical помощью Electrophoresis (PACE).
  • To showcase PACE as a sensitive and straightforward technique for polysaccharide investigation.

Main Methods:

  • Utilizes derivatization of reducing ends of sugars with a fluorophore.
  • Employs electrophoresis under optimized conditions within polyacrylamide gels.

Main Results:

  • PACE provides a sensitive and simple approach for polysaccharide analysis.
  • Demonstrates applicability in determining polysaccharide structure and quantity.

Conclusions:

  • PACE is a valuable tool for polysaccharide research.
  • The technique is effective for investigating enzyme specificity related to polysaccharides.