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Updated: Dec 16, 2025

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Published on: February 27, 2021
Electron Tomography and Immunogold Labeling as Tools to Analyze De Novo Assembly of Plant Cell Walls
Marisa S Otegui1,2,3
1Department of Botany, University of Wisconsin, Madison, WI, USA. otegui@wisc.edu.
Abstract:
High-resolution imaging of the membranous intermediates and cytoskeletal arrays involved in the assembly of a new cell wall during plant cytokinesis requires state-of-the-art electron microscopy techniques. The combination of cryofixation/freeze-substitution methods with electron tomography (ET) has revealed amazing structural details of this unique cellular process. This chapter deals with the main steps associated with these imaging techniques: selection of samples suitable for studying plant cytokinesis, sample preparation by high-pressure freezing/freeze substitution, and ET of plastic sections. In addition, immunogold approaches for identification of proteins and polysaccharides during cell wall assembly are discussed.
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