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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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An open-hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses.

C T Russell^1,2, E J Rees¹

¹Department of Chemical Engineering and Biotechnology, Cambridge University, Cambridge, U.K.

Journal of Microscopy

|July 4, 2020

Summary

This summary is machine-generated.

We developed an open-hardware sample mounting system for light-sheet microscopy. This 3D-printed system overcomes limitations with standard mounting methods, enabling effective live biological sample imaging.

Keywords:

cell imaging light-sheet microscopy organismal imaging sample mounting volumetric imaging

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Area of Science:

Biophysics
Microscopy Engineering

Background:

Standard sample mounting methods are often incompatible with light-sheet microscopy setups.
Orthogonal illumination and detection in light-sheet microscopy limit sample positioning and cover slip dimensions.

Purpose of the Study:

To present an open-hardware sample mounting system for light-sheet microscopes.
To overcome existing constraints in sample positioning and mounting for light-sheet microscopy, particularly with large detection objectives.

Main Methods:

Designed and constructed an open-hardware sample chamber using 3D printed components.
Integrated the custom sample chamber into a light-sheet microscope setup.
Tested the system's efficacy using live biological samples.

Main Results:

The developed system accommodates large detection objectives.
Demonstrated successful imaging of live biological samples within the custom chamber.
The 3D-printed system provides a viable alternative to conventional mounting techniques.

Conclusions:

The open-hardware sample mounting system effectively addresses limitations in light-sheet microscopy sample preparation.
This innovative approach enhances compatibility with large detection objectives and standard sample mounting.
The system's efficacy is validated through live biological sample imaging.