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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Dec 16, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Technologies and Computational Analysis Strategies for CRISPR Applications.

Kendell Clement1, Jonathan Y Hsu1, Matthew C Canver2

  • 1Molecular Pathology Unit, Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA; Department of Pathology, Harvard Medical School, Boston, MA, USA; The Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Molecular Cell
|July 4, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas technology enables precise eukaryotic genome and epigenome editing for biological research and therapeutics. Computational tools are advancing the analysis of on-target and off-target CRISPR editing and pooled screen data.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • CRISPR-Cas systems provide a programmable platform for eukaryotic genome and epigenome editing.
  • Targeted genetic and epigenetic perturbations are crucial for basic biology research and therapeutic development.

Purpose of the Study:

  • To review key computational advances and considerations for CRISPR applications.
  • To focus on the analysis of on- and off-target editing and CRISPR pooled screen data.

Main Methods:

  • Review of available CRISPR technologies.
  • Analysis of computational approaches for CRISPR applications.
  • Examination of assays for off-target editing identification.

Main Results:

  • CRISPR systems have been engineered for increased precision, efficiency, and flexibility in DNA and RNA targeting.
  • Comprehensive and sensitive assays for off-target editing are becoming available.
  • High-throughput genome and epigenome editing techniques paired with various readouts are uncovering cellular functions.

Conclusions:

  • Technological advances in CRISPR editing are driven by computational approaches.
  • Computational tools are essential for analyzing CRISPR on- and off-target editing and pooled screen data.
  • CRISPR technology holds significant potential for basic research and novel therapeutic development.