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Related Experiment Video

Updated: Dec 16, 2025

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Which pathologic staining method can visualize the hyperacute infarction lesion identified by diffusion MRI?: A

Kyung Sik Yi1, Chi-Hoon Choi1, Cheolkyu Jung2

  • 1Department of Radiology, Chungbuk National University Hospital, Cheongju, Chungbuk, Republic of Korea.

Journal of Neuroscience Methods
|July 4, 2020
PubMed
Summary
This summary is machine-generated.

Hematoxylin and eosin (H&E) staining effectively delineates hyperacute infarction lesions on diffusion-weighted MRI (DWI). This method provides superior histopathological identification compared to other stains for early stroke assessment.

Keywords:
Diffusion-weighted imagingExperimental animal modelImmunohistochemistryMCA infarctionPathologyStroke

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Area of Science:

  • Neuropathology
  • Neuroimaging
  • Histology

Background:

  • Hyperacute infarction identification is crucial for timely stroke intervention.
  • Diffusion-weighted MRI (DWI) detects early ischemic changes.
  • Accurate histopathological correlation of DWI findings is needed.

Purpose of the Study:

  • To develop a staining method for delineating macroscopic lesion boundaries of hyperacute infarction on DWI.
  • To validate the infarction boundary using various staining techniques.
  • To compare the efficacy of different stains in identifying early ischemic lesions.

Main Methods:

  • Five staining methods (H&E, Nissl, TTC, MAP2, TUNEL) were applied to rat MCA infarction models.
  • Lesion areas from DWI were compared with histopathological findings using Dice indices.
  • Regions of interest (ROIs) were categorized based on staining abnormalities (ROIA, ROIB, ROIC).

Main Results:

  • Hematoxylin and eosin (H&E) staining showed significantly higher Dice indices compared to Nissl and MAP2 stains (p=0.000).
  • H&E staining best reflected the 1-hour hyperacute DWI abnormal lesions.
  • Microscopic evaluation revealed the most severe cellular damage in ROIA.

Conclusions:

  • H&E staining enables macroscopic delineation of 1-hour DWI-abnormal lesions.
  • MAP2 and Nissl stains only partially depicted the infarction lesions.
  • H&E staining is a reliable method for histopathological validation of early ischemic changes.