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A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study

Charan Kumar V Devarakonda1, Emily R Shearier1, Chaoran Hu2

  • 1Center for Vascular Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT, 06030, USA.

Journal of Pediatric Urology
|July 5, 2020
PubMed

Insights

New urinary biomarker proteins were identified to reliably detect ureteropelvic junction obstruction (UPJO) in children. This panel of proteins offers a promising tool for diagnosing obstructive uropathy in infants.

Area of Science:

  • Biochemistry
  • Pediatric Nephrology
  • Biomarker Discovery

Background:

  • Ureteropelvic junction obstruction (UPJO) diagnosis in children lacks consistent urinary biomarkers.
  • Existing protein biomarkers show variable predictive accuracy, necessitating improved diagnostic tools.

Purpose of the Study:

  • To identify and validate a panel of reliable urinary biomarker proteins for diagnosing UPJO in pediatric patients.
  • To establish a consistent and accurate method for detecting obstructive uropathy in infants.

Main Methods:

  • Mass spectrometry analysis of urine from infants with and without UPJO.
  • Proteins preferentially identified in UPJO samples were selected and ranked by diagnostic odds ratio.
  • Top candidate proteins were validated using ELISA, with results normalized to urine creatinine.

Main Results:

  • Five proteins (prostaglandin-reductase-1, ficolin-2, nicotinate-nucleotide pyrophosphorylase, immunoglobulin superfamily-containing leucine-rich-repeat-protein, vascular cell adhesion molecule-1) were identified at higher levels in UPJO samples.
  • The fold-change of median protein concentrations ranged from 2.9 to 9.4.
  • A prevalence order of PTGR1>FCN2>QPRT>ISLR>VCAM1 was observed in UPJO samples.

Conclusions:

  • A novel panel of urinary proteins can reliably distinguish between obstructed and non-obstructed infants.
  • This biomarker panel holds potential for diagnosing obstructive uropathy and may be applicable to other diseases.
Abstract

Keywords:
BiomarkerFicolin-2 (FCN2)Immunoglobulin superfamily containing leucine-rich repeat protein (ISLR)Nicotinate-nucleotide pyrophosphorylase [carboxylating] (QPRT)Prostaglandin reductase 1 (PTGR1)Ureteropelvic junction obstruction

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