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Related Concept Videos

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The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
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Updated: Dec 16, 2025

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Orally administrated chitosan microspheres bind Helicobacter pylori and decrease gastric infection in mice.

Patrícia C Henriques1, Lia M Costa2, Catarina L Seabra3

  • 1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.

Acta Biomaterialia
|July 5, 2020
PubMed
Summary
This summary is machine-generated.

Chitosan microspheres effectively reduce Helicobacter pylori infection in mice, offering a promising antibiotic-free treatment. These microspheres bind and remove H. pylori, demonstrating potential for gastric cancer prevention.

Keywords:
Bacteria adhesionBiomaterialsIn vivoMicroparticlesMucoadhesion

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Area of Science:

  • Biomaterials Science
  • Gastroenterology
  • Infectious Diseases

Background:

  • Persistent Helicobacter pylori infection is a major cause of gastric cancer, affecting over 50% of the global population.
  • Rising antibiotic resistance and treatment inefficiencies necessitate alternative therapeutic strategies for H. pylori eradication.
  • H. pylori's ability to adhere to gastric mucus and reside within foveolae complicates eradication efforts.

Purpose of the Study:

  • To evaluate the biocompatibility, mucopenetration, and H. pylori treatment efficacy of chitosan microspheres (ChMics) after oral administration.
  • To investigate the influence of microsphere size and chitosan degree of acetylation on H. pylori binding and removal.
  • To assess the potential of ChMics as an antibiotic-free therapeutic approach for H. pylori infection.

Main Methods:

  • Chitosan microspheres of varying sizes (XL, XS) and degrees of acetylation (6%, 16%) were synthesized.
  • In vitro cytotoxicity assays were performed on human gastric cells.
  • Ex vivo mucopenetration studies and in vivo H. pylori infection reduction assays in C57BL/6 mice were conducted.

Main Results:

  • ChMics demonstrated adherence to H. pylori strains without exhibiting cytotoxicity to gastric cells.
  • Smaller microspheres (XS) showed enhanced penetration into gastric foveolae.
  • Oral administration of mucoadhesive ChMics (XL6, XS6) resulted in an 88% reduction in H. pylori infection in mice.
  • Chitosan degree of acetylation was a more significant factor than microsphere size in H. pylori removal efficiency.

Conclusions:

  • Chitosan microspheres are a viable antibiotic-free strategy for reducing H. pylori gastric infection.
  • Microsphere properties, particularly degree of acetylation, can be tuned to optimize H. pylori binding and removal.
  • This approach holds significant potential for clinical application in combating H. pylori-associated diseases.