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Updated: Jan 24, 2026

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
Published on: October 30, 2017
[Production kinetics of T-lymphocyte activity regulating human granulopoiesis in vitro]
T Bonnefoix1, M P Piccinni, J J Sotto
1Service d'Hématologie, CHUR, Grenoble, France.
Abstract:
Human T-lymphocytes stimulated with Con A are able to produce both a granulocyte-colony-stimulating activity (G-CSA) and a colony-inhibiting activity. We have studied here the cumulative and daily non-cumulative production of these 2 activities. The release of G-CSA is low on the 1st day of culture, maximal production occurred between the 2nd and the 4th day; from the 6th day on, T-lymphocytes are unable to produce CSA. Colony-inhibiting activity production is usually weak at the beginning of culture, but constantly found on day 5 and on day 7. In conclusion, cumulative culture for 5 days seems optimal for the simultaneous detection of both activities.
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