Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Model for tissue specific Calcitonin/CGRP-I RNA processing from in vitro experiments.

R A Bovenberg1, G J Adema, H S Jansz

  • 1Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.

Nucleic Acids Research
|August 25, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MitoTam induces ferroptosis and increases radiosensitivity in head and neck cancer cells.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2024
Same author

Radiotherapy induces an increase in serum antioxidant capacity reflecting tumor response.

Clinical and translational radiation oncology·2024
Same author

Tamoxifen induces radioresistance through NRF2-mediated metabolic reprogramming in breast cancer.

Cancer & metabolism·2023
Same author

Sialic acid blockade in dendritic cells enhances CD8<sup>+</sup> T cell responses by facilitating high-avidity interactions.

Cellular and molecular life sciences : CMLS·2022
Same author

Generation of αCD11b-CpG antibody conjugates for the targeted stimulation of myeloid cells.

Journal of controlled release : official journal of the Controlled Release Society·2021
Same author

A systems genomics approach identifies <i>SIGLEC15</i> as a susceptibility factor in recurrent vulvovaginal candidiasis.

Science translational medicine·2019

The Calcitonin/CGRP-I (CALC-I) gene produces distinct mRNAs in different cells. Tissue-specific expression is primarily regulated by polyadenylation site cleavage, not just splicing, influencing Calcitonin and CGRP-I mRNA production.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The Calcitonin/CGRP-I (CALC-I) gene exhibits tissue-specific expression, yielding Calcitonin mRNA in thyroid C-cells and CGRP-I mRNA in nerve cells.
  • Alternative RNA processing, including splicing and polyadenylation, dictates the final mRNA products.

Purpose of the Study:

  • To investigate the in vitro RNA processing mechanisms of the human CALC-I gene.
  • To identify the key regulatory steps controlling tissue-specific Calcitonin and CGRP-I mRNA production.

Main Methods:

  • Utilized a model precursor RNA spanning exons 3-5 of the human CALC-I gene.
  • Performed in vitro splicing and polyadenylation assays using nuclear extracts from Hela, PC12, and Ewing-1B cells.

Main Results:

Related Experiment Videos

  • CGRP mRNA-specific splicing (exon 3 to exon 5) was predominant across all tested cell extracts (Hela, PC12, Ewing-1B).
  • Calcitonin mRNA-specific splicing (exon 3 to exon 4) occurred significantly only after eliminating the downstream CGRP splice site.
  • Cleavage at the Calcitonin mRNA-specific poly (A) site at exon 4 was identified as a critical regulatory event.

Conclusions:

  • Tissue-specific expression of the CALC-I gene is predominantly regulated by polyadenylation site choice, specifically cleavage at the exon 4 poly (A) site.
  • This polyadenylation-coupled regulation influences the balance between Calcitonin and CGRP-I mRNA production.