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Microbial potentiometric sensor: A new approach to longstanding challenges.

Scott R Burge1, Kiril D Hristovski2, Russell G Burge1

  • 1Burge Environmental Inc., 6100 South Maple Avenue, Suite 114, Tempe, AZ 85283, USA.

The Science of the Total Environment
|July 6, 2020
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Summary

Microbial Potentiometric Sensor (MPS) systems provide accurate, long-term environmental monitoring. These sensors demonstrate durability and reliability, outperforming traditional sensors in wastewater treatment analysis.

Keywords:
BiofilmLong-term monitoringMicrobial potentiometric sensorReal-time monitoring

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Microbiology

Background:

  • Traditional environmental monitoring often lacks long-term, continuous spatial and temporal resolution.
  • Microbial processes are crucial in environmental systems but challenging to monitor continuously.
  • Simple potentiometric measurements offer a potential avenue for improved environmental sensing.

Purpose of the Study:

  • To test the hypothesis that Microbial Potentiometric Sensor (MPS) systems can resolve spatial and temporal changes in environmental systems through long-term, continuous potentiometric measurements.
  • To evaluate the performance, accuracy, reproducibility, and durability of MPS systems under laboratory and field conditions.
  • To compare the efficacy of MPS signals against traditional sensors like dissolved oxygen (DO) and oxidation-reduction potential (ORP) in environmental and wastewater treatment applications.

Main Methods:

  • Development and conceptual description of the Microbial Potentiometric Sensor (MPS) system.
  • Laboratory-based performance testing of MPS systems, including accuracy, reproducibility, and correlation with DO and ORP.
  • Field deployment of MPS systems in a model wetland and a batch-wastewater treatment facility for long-term monitoring (>2 years).
  • Analysis of MPS signal patterns and magnitudes to assess environmental changes and process correlations.

Main Results:

  • MPS systems exhibited high accuracy and reproducibility in laboratory settings, with significant correlations to DO and ORP.
  • MPS sensors operated continuously for over two years without interruption or cleaning in a model wetland.
  • In wastewater treatment, MPS signals effectively described organic carbon trends and correlated with treatment phases, outperforming DO and ORP sensors.
  • MPS signals demonstrated reliability and reproducibility, providing better insights into carbon treatment levels.

Conclusions:

  • Microbial Potentiometric Sensor (MPS) systems are suitable for long-term, continuous monitoring of environmental systems.
  • The durability and accuracy of MPS technology make it a valuable tool for environmental and wastewater process analysis.
  • Analyzing both the magnitude and pattern of MPS signals provides comprehensive insights for optimizing microbial processes and environmental conditions.