Reference values for a novel ambulatory-based frequency domain T-wave alternans in subjects without structural heart...

Conventional frequency domain T wave alternans (FD-TWA) is a noninvasive risk stratification marker for identifying arrhythmic sudden cardiac death, but the conventional FD-TWA device that was considered the gold standard device has been discontinued commercially. Recently, a newly developed ambulatory electrocardiogram (AECG) device that can detect FD-TWA continuously for 24 hours is available in clinical settings. However, information on the normal values using the novel AECG-based frequency domain TWA (FD-TWA) is lacking.

FD-TWA for AECG was examined in 312 subjects without heart disease (Sb-wHD) (range 20-89 years, 146 men) and 30 heart disease patients (HD-P) (mean age 57±17 years, 24 men). The maximum FD-TWA amplitude over 24 hours was measured with manual editing. The upper limit of local noise levels for measurement of FD-TWA was set to both <10 μV and <20 μV (acceptable noise level <10 μV and <20 μV).

The reference values (95th percentiles) of FD-TWA in Sb-wHD were 19.9 μV for the acceptable noise level <10 μV and 23.6 μV for the acceptable noise level <20 μV. The 75th percentile of FD-TWA amplitude in HD-P was 19.5 µV at an acceptable noise level <10 µV and 21.5 µV at an acceptable noise level <20 µV. FD-TWA amplitude without heart disease was significantly affected by heart rate when the maximum FD-TWA was measured (β = 0.274 p < 0.001 for the acceptable noise level <10 μV; β = 0.263, p < 0.001 for the acceptable noise level <20 μV) and age (β = 0.204, p = 0.004 for the acceptable noise level <10 μV; β = 0.149, p = 0.034 for the acceptable noise level <20 μV).

In the present study, the reference values for a novel FD-TWA in Sb-wHD and the distribution of TWA values in HD-P were established. In future research, the cut-off values of FD-TWA in HD-P will need to be examined.