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Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration
Andreas Tosstorff1, Cora Kroner1, Diederik J Opperman2
1DECHEMA Research Institute Frankfurt Germany.
Abstract:
Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.
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