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PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
Published on: December 27, 2013
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Protein-based nanoformulations for α-tocopherol encapsulation.
Fatemeh Shahmoradi Ghaheh1, Akbar Khoddami1, Farzaneh Alihosseini1
1Department of Textile Engineering Isfahan University of Technology Isfahan Iran.
Engineering in Life Sciences
|July 7, 2020
Summary
Biodegradable nanoparticles encapsulating alpha-tocopherol (vitamin E) were created using BSA and silk fibroin. These non-toxic nanoformulations show antioxidant activity and are suitable for skincare and textile applications.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Alpha-tocopherol (vitamin E) is a potent antioxidant with therapeutic applications.
- Developing effective delivery systems for lipophilic compounds like alpha-tocopherol is crucial for enhanced bioavailability and efficacy.
- Biodegradable and non-toxic protein-based nanoparticles offer promising platforms for drug and cosmetic ingredient delivery.
Purpose of the Study:
- To develop and characterize novel nanoparticles using bovine serum albumin (BSA) and silk fibroin (SF) for entrapping alpha-tocopherol (α-VO).
- To evaluate the encapsulation efficiency, particle size, surface charge, cytotoxicity, and antioxidant properties of the developed nanoformulations.
- To assess the potential of these nanoformulations for functionalizing textiles and for controlled delivery of α-VO into the skin.
Main Methods:
- Nanoparticles were synthesized using ultrasonic emulsification with BSA and SF as carriers for α-VO.
- Particle size and surface charge were analyzed using dynamic light scattering (DLS).
- Encapsulation efficiency was determined, and cytotoxicity was assessed using human skin keratinocytes. Free radical scavenging activity was evaluated using the ABTS assay.
Main Results:
- The produced nanoparticles exhibited a particle size of 200-300 nm and a highly negative surface charge.
- Encapsulation efficiencies for α-VO were approximately 99%, indicating effective loading.
- The nanoformulations demonstrated significant free radical scavenging ability and showed no significant cytotoxicity towards human skin keratinocytes.
Conclusions:
- BSA and SF can effectively encapsulate α-VO into stable, biodegradable nanoparticles.
- These nanoformulations are non-toxic and possess antioxidant properties, making them suitable for cosmetic and textile applications.
- The developed nanodevices represent a promising approach for the controlled delivery of α-VO and for creating functionalized textiles.
