DBT desulfurization by decorating Rhodococcus erythropolis IGTS8 using magnetic Fe₃O₄ nanoparticles in a bioreactor

Today, crude oil is an important source of energy and environmental contamination due to the continued use of petroleum products is a matter or urgent concern. In this work, two technological platforms, namely, the use of a robust desulfurizing bacteria and the use of nanotechnology to decorate the surface of the bacteria with nanoparticles (NP), were combined to enhance biodesulfurization (BDS). BDS is an ecologically friendly method for desulfurizing petroleum products while avoiding damage to the hydrocarbons due to the high temperatures normally associated with physical desulfurization methods. First, a bacterium known to be a good organism for desulfurization (Rhodococcus erythropolis IGTS8) was employed in cell culture to remove a recalcitrant sulfur molecule from a common sulfur-containing compound found in crude petroleum products (dibenzothiophene). 2-Hydroxybiphenyl (2-HBP) produced as a consequence of the BDS of dibenzothiophene was determined using Gibbs' assay. The synthesized NP were characterized by field emission scanning electron microscope, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, and vibrating sample magnetometer. The field emission scanning electron microscope and transmission electron microscopy images showed the size of the NP is 7-8 nm. The decorated cells had a long lag phase, but the growth continued until 148 h (at OD₆₀₀ = 3.408) while the noncoated bacteria grow until 96 h before entering the stationary phase at OD₆₀₀ = 2.547. Gibbs' assay results showed that production of 2-HBP by decorated cells was 0.210 mM at t = 148 h, while 2-HBP production by nondecorated cells was 0.182 mM at t = 96 h. Finally, the experiments were repeated in a fermenter.