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Monitoring Protein Adsorption with Solid-state Nanopores
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Protein adsorption onto monoliths: A surface energetics study.

Muhammad Aasim1, Muhammad Hidayatullah Khan1,2, Noor Shad Bibi3

  • 1Department of Biotechnology University of Malakand Chakdara Dir (Lower) Pakistan.

Engineering in Life Sciences
|July 7, 2020
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Summary

This study explores protein adsorption chromatography using surface energetics. Butyl ligands show stronger protein interaction than phenyl ligands, impacting downstream processing efficiency.

Keywords:
Energy minimumMonoliths with butylMonoliths with phenylSurface energetics approach

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Area of Science:

  • Chromatography
  • Bioseparation
  • Surface Science

Background:

  • Adsorption chromatography is crucial for protein purification.
  • Understanding protein-material interactions is key for optimizing separation processes.

Purpose of the Study:

  • To investigate the interaction of model proteins with monolithic chromatographic materials.
  • To explore the influence of butyl and phenyl ligand densities on protein-monolith interactions using a surface energetics approach.

Main Methods:

  • Characterization of protein and monolith physicochemical properties via contact angle and zeta potential.
  • Application of surface energetics to calculate protein-monolith interaction energy at varying conditions.
  • Analysis of operating parameters including ligand density, salt concentration, and lambda.

Main Results:

  • Monolith hydrophobicity increases with ligand density, enhancing interaction strength.
  • Proteins exhibited higher interaction energy with butyl ligands compared to phenyl ligands.
  • Lactoferrin interaction energy was 24.38 kT with butyl monoliths versus 23.28 kT with phenyl monoliths (100 mM ammonium sulphate).

Conclusions:

  • Butyl ligands result in stronger protein immobilization, requiring more energy and time for elution.
  • Findings provide fundamental insights into protein-monolith interactions for downstream processing design.
  • This research aids in developing efficient and cost-effective bioseparation strategies.