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Microbial Fermentation01:23

Microbial Fermentation

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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Related Experiment Video

Updated: Dec 16, 2025

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

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The ejector loop reactor: Application for microbial fermentation and comparison with a stirred-tank bioreactor.

Manuel Ughetti1,2, Daniel Jussen1, Peter Riedlberger1

  • 1Research Group Chemical Engineering Institute of Chemistry and Biotechnology Zurich University of Applied Sciences Wädenswil Switzerland.

Engineering in Life Sciences
|July 7, 2020
PubMed
Summary

Ejector loop reactors (ELRs) show potential for microbial fermentation, yielding higher cell densities than stirred-tank reactors (STRs). While mass transfer is comparable, ELRs caused increased cell disruption, indicating areas for optimization in bioreactor design.

Keywords:
Ejector loop reactorEscherichia coliMicrobial FermentationOxygen transfer rateStirred‐tank reactor

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Ejector loop reactors (ELRs) offer superior mass transfer rates in gas/liquid reactions compared to stirred-tank reactors (STRs).
  • Oxygen transport limitations and shear stress can inhibit microbial growth and recombinant protein production during fermentation.
  • The Buss-Loop® Reactor (BLR), an advanced ELR, was investigated for its suitability as a bioreactor.

Purpose of the Study:

  • To evaluate the Buss-Loop® Reactor (BLR) as a bioreactor for microbial fermentation.
  • To compare the BLR's performance against a conventional stirred-tank reactor (STR).
  • To assess the impact of ELR's shear stress on biomass and recombinant protein production.

Main Methods:

  • Lab-scale Buss-Loop® Reactor (BLR) adapted for microbial fermentation.
  • Comparison with a reference stirred-tank reactor (STR).
  • Measurement of mass transfer rates, specific power input, cell density (OD600), and green fluorescence protein (GFP) production in *Escherichia coli* K12 MG1655.

Main Results:

  • Comparable mass transfer rates and specific power inputs were observed between the BLR and STR.
  • Maximum cell densities were higher in the BLR (OD600 of 22) than in the STR (OD600 of 18).
  • Green fluorescence protein (GFP) production was comparable, but higher release into the media was observed in the BLR, suggesting increased cell disruption.

Conclusions:

  • The ejector loop reactor (ELR) demonstrates significant potential for enhancing microbial fermentation processes.
  • Higher biomass yields in ELRs are achievable, though cell disruption requires further investigation and mitigation.
  • The BLR shows promise as an alternative bioreactor system, particularly for applications benefiting from enhanced mass transfer.