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Updated: Dec 16, 2025
Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
Published on: March 3, 2020
A one-pot process for synthesis of mitomycin analogs catalyzed by laccase/lipase optimized by response surface
Yuanyuan Zhang1,2, Quancai Yao1, Zewen Li1
1Department of Pharmaceutical Engineering in College of Chemical Engineering Qingdao University of Science and Technology Qingdao P. R. China.
Abstract:
To reach the excellent yield as well as environmental friendliness, an efficient one-pot process for the synthesis of 2-methyl-3-n-butylaminoyl-1,4-benzoquinone, a mitomycin-like compound by the domino reaction of 2-methyl-1,4-hydroquinone and butylamine using laccase/lipase as co-catalysts, has been developed. In this present study, the process proposed here was further improved by optimizing the relevant factors using the response surface methodology based on Box-Benkhen Design. The optimum condition that afforded the highest yield (98%) of 2-methyl-3-n-butylaminoyl-1,4-benzoquinone was obtained as follows: molar ratio of amines to hydroquinones 1.16:1, activity ratio of laccase to lipase 1.14:2, and reaction temperature 38.9°C. The results obtained indicate that this process may be useful as a green alternative method for higher yield production of mitomycin analogs.
