Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autoimmune Disorders01:29

Autoimmune Disorders

1.3K
Autoimmune diseases are a group of disorders in which the body's immune system mistakenly attacks its own cells, tissues, and organs. This results from an overactive immune response against substances and tissues normally present in the body. Let's delve into the concept and mechanism of autoimmune diseases from an immune system point of view, explore different causes and examples of such diseases, and discuss potential solutions.
Concept and Mechanism of Autoimmune Diseases
The immune...
1.3K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.6K
Pathophysiology of Diabetes01:20

Pathophysiology of Diabetes

2.9K
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
2.9K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

8.2K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
8.2K
Diabetes Mellitus: Overview and Type I Subtype01:22

Diabetes Mellitus: Overview and Type I Subtype

4.7K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
4.7K
What is the Immune System?01:38

What is the Immune System?

125.5K
Overview
125.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic impact of bone marrow iron and serum ferritin in patients with myelodysplastic syndromes.

Annals of hematology·2026
Same author

Repurposing ALK inhibitors as influenza and corona virus antivirals targeting lymphocyte tyrosine kinase (LTK).

Virus research·2026
Same author

eIF3e-mediated translational checkpoint maintains immune tolerance and prevents lymphoid malignancy.

The Journal of experimental medicine·2026
Same author

RNA helicase DHX29 controls the translation of transcription factors involved in germinal center response and plasma cell differentiation in mice.

The EMBO journal·2026
Same author

Associations of short-term exposure to PM<sub>2.5</sub> and its metal constituents with fasting plasma glucose in older adults: The mediating role of systemic inflammation.

Ecotoxicology and environmental safety·2026
Same author

Existence of Causation without Correlation in Transcriptional Networks.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Dec 16, 2025

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
06:27

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

17.1K

Prediabetes Induced by a Single Autoimmune B Cell Clone.

Nathaniel Phillips1, Eugene Ke2, Amy Nham1

  • 1Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, United States.

Frontiers in Immunology
|July 7, 2020
PubMed
Summary

This study developed a novel mouse model to investigate B cells in type-1 diabetes (T1D). The model revealed spontaneous immune responses and impaired glucose tolerance, offering new insights into T1D pathogenesis.

Keywords:
B cellanimal modelautoimmunitycloningdiabetesnucleic acid sensingpancreassomatic cell nuclear transfer (SCNT)

More Related Videos

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
10:03

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Published on: November 18, 2022

2.1K
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
11:31

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Published on: August 16, 2019

8.3K

Related Experiment Videos

Last Updated: Dec 16, 2025

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
06:27

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Published on: May 6, 2013

17.1K
Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
10:03

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Published on: November 18, 2022

2.1K
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
11:31

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Published on: August 16, 2019

8.3K

Area of Science:

  • Immunology
  • Endocrinology
  • Genetics

Background:

  • B cells are implicated in type-1 diabetes (T1D) onset.
  • Their precise role during early T1D stages remains largely unknown.

Purpose of the Study:

  • To investigate the function of early pancreas-infiltrating B cells in T1D.
  • To develop a novel mouse model for studying B cell involvement in T1D.

Main Methods:

  • Somatic Cell Nuclear Transfer (SCNT) was used to create a novel B cell receptor (BCR) mouse model (B1411).
  • RNA-Sequencing (RNA-Seq) was performed on B1411 cells.
  • B1411-Rag1-/- mice were challenged with glucose.

Main Results:

  • The B1411 model exhibited spontaneous germinal center reactions and class-switching without T cell help.
  • RNA-Seq identified differentially expressed genes, including Irf7, Usp18, Mda5, and OAS family members (e.g., Oas1), linked to T1D.
  • B1411-Rag1-/- mice showed impaired glucose tolerance when challenged with glucose.

Conclusions:

  • The novel B1411 mouse model provides new insights into B cell roles in early T1D.
  • Gene expression patterns suggest potential links between viral etiology and T1D pathogenesis.
  • The model demonstrates impaired glucose tolerance, highlighting B cell impact on metabolic function in T1D.