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Diosgenin Exerts Antitumor Activity via Downregulation of Skp2 in Breast Cancer Cells

Yanling Liu1, Zijun Zhou2, Jingzhe Yan3

  • 1Department of Breast Medicine, Jilin Province Cancer Hospital, Changchun 130012, China.

Biomed Research International
|July 7, 2020
PubMed
Abstract

Insights

Diosgenin, a natural compound, shows promise in fighting breast cancer by inhibiting S-phase kinase-associated protein 2 (Skp2). This study demonstrates diosgenin

Area of Science:

  • Oncology
  • Molecular Biology
  • Natural Products Chemistry

Background:

  • Breast cancer is a leading cause of mortality in women.
  • S-phase kinase-associated protein 2 (Skp2) promotes breast cancer growth.
  • Targeting Skp2 presents a potential therapeutic strategy.

Purpose of the Study:

  • To investigate the antitumor activity of diosgenin in breast cancer.
  • To determine if diosgenin inhibits Skp2 expression and function.

Main Methods:

  • MTT assay for cell viability.
  • Transwell invasion assay for cell motility.
  • RT-PCR and Western blotting for gene and protein expression analysis.
  • Transfection techniques.

Main Results:

  • Diosgenin inhibited breast cancer cell viability and induced apoptosis.
  • Diosgenin reduced cancer cell invasion and motility.
  • Diosgenin suppressed Skp2 expression in breast cancer cells.

Conclusions:

  • Diosgenin exhibits antitumor effects in breast cancer cells.
  • Diosgenin's mechanism involves the inhibition of Skp2.
  • Diosgenin is a potential therapeutic agent for breast cancer treatment.

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