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Related Experiment Videos

Receptors for T cell-replacing factor/interleukin 5. Specificity, quantitation, and its implication.

S Mita1, N Harada, S Naomi

  • 1Department of Biology, Kumamoto University Medical School, Japan.

The Journal of Experimental Medicine
|September 1, 1988
PubMed
Summary

Researchers identified the Interleukin-5 receptor (IL-5-R) on B cells using radiolabeled IL-5. This receptor is crucial for B cell growth and differentiation, particularly in activated B cells, mediating IL-5

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Interleukin-5 (IL-5), also known as T cell-replacing factor (TRF), is a key cytokine for B cell development and function.
  • The mechanism of IL-5 action is mediated through specific cell surface receptors, but these have not been fully characterized.

Purpose of the Study:

  • To characterize the binding of IL-5 to cells and identify its specific cell surface receptor.
  • To investigate the expression of the IL-5 receptor on various cell types and its role in B cell differentiation.

Main Methods:

  • Biosynthetically [35S]methionine-labeled IL-5 and 125I-IL-5 were used to study IL-5 binding.
  • Radioligand binding assays were performed on BCL1-B20 cells and other cell lines.
  • Crosslinking studies identified the molecular weight of the IL-5 receptor subunit.

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Main Results:

  • IL-5 binds specifically to BCL1-B20 cells with high (Kd = 66 pM) and low (Kd = 12 nM) affinity binding sites.
  • A membrane polypeptide of Mr 46,500 was identified as the IL-5 receptor subunit.
  • IL-5 receptor expression was detected on activated B cells and some early B cell lines, but not on T cells, mast cells, or macrophages.

Conclusions:

  • The high-affinity IL-5 receptor binding sites mediate the biological effects of IL-5.
  • Identification and characterization of the IL-5 receptor provide insights into IL-5's diverse biological activities.
  • IL-5 receptor expression on activated B cells correlates with IL-5's role in B cell differentiation into Ig-secreting cells.