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Related Experiment Video

Updated: Dec 16, 2025

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Maintenance of Primary Human Colorectal Cancer Microenvironment Using a Perfusion Bioreactor-Based 3D Culture System.

Celeste Manfredonia1,2, Manuele G Muraro2,3, Christian Hirt2,3

  • 1Cancer Immunotherapy, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 4031, Switzerland.

Advanced Biosystems
|July 7, 2020
PubMed
Summary
This summary is machine-generated.

A novel bioreactor system preserves key tumor microenvironment cells in colorectal cancer (CRC) tissue. This 3D culture method accurately predicts patient-specific drug responses, improving cancer therapy development.

Keywords:
3D modelcolorectal cancerhuman tumor tissue cultureimmune cellsperfusion bioreactortissue microenvironment

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Area of Science:

  • Oncology
  • Biotechnology
  • Tissue Engineering

Background:

  • Colorectal cancer (CRC) remains a leading cause of cancer mortality.
  • Current in vitro models inadequately represent the tumor microenvironment (TME), limiting novel therapy development.
  • The TME, including stromal and immune cells, significantly influences CRC progression and drug sensitivity.

Purpose of the Study:

  • To evaluate a bioreactor-based 3D culture system for preserving TME components in primary CRC samples.
  • To determine if this system can maintain tissue architecture and cellular viability.
  • To assess the suitability of the system for predicting patient-specific drug responses.

Main Methods:

  • Primary CRC tissue fragments were cultured in a sandwich format between collagen scaffolds within a bioreactor.
  • Cultures were maintained under static or perfused conditions for up to 3 days.
  • Tissue architecture, cell densities, stromal/immune cell viability, and drug sensitivity were assessed.

Main Results:

  • Perfused cultures significantly better maintained tumor tissue architecture and proliferating cell densities compared to static cultures.
  • Stromal and immune cells remained viable and responsive to stimuli in perfused cultures.
  • The perfusion-based system was suitable for testing primary CRC cell sensitivity to standard chemotherapies.

Conclusions:

  • Bioreactor-based perfusion culture effectively preserves the cellular composition and architecture of the CRC TME.
  • This patient-specific 3D culture model shows promise for evaluating drug sensitivity in colorectal cancer.
  • The system facilitates the development of more effective and personalized CRC treatment strategies.