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Long noncoding RNA GAS5 enhanced by curcumin relieves poststroke depression by targeting miR-10b/BDNF in rats
1Department of Neurology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai, University of Traditional Chinese Medicine.
Summary
Curcumin effectively treats post-stroke depression (PSD) in rats by modulating the GAS5/miR-10b/BDNF pathway. This natural compound alleviates depressive symptoms and impacts key molecular signaling involved in brain recovery.
Area of Science:
- Neuroscience
- Pharmacology
- Molecular Biology
Background:
- Post-stroke depression (PSD) treatment typically involves combined anti-stroke and antidepressant medications.
- Understanding novel therapeutic mechanisms for PSD is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate the therapeutic effects of curcumin on post-stroke depression (PSD) in a rat model.
- To elucidate the underlying molecular mechanisms, focusing on the GAS5/miR-10b/BDNF regulatory axis.
Main Methods:
- Behavioral tests (open field, tail suspension) assessed depression-like behaviors in rats.
- Serum levels of corticosterone and CRH were measured.
- Hippocampal neurotransmitter levels (5-HT, 5-HIAA, DA) and gene/protein expression (GAS5, miR-10b, BDNF) were analyzed.
Main Results:
- Curcumin treatment significantly alleviated depressive symptoms in PSD rats compared to controls.
- Curcumin upregulated long noncoding RNA GAS5, which activated the BDNF/Trkβ signaling pathway.
- GAS5 acted as a sponge for miR-10b, reducing miR-10b levels and consequently impacting BDNF mRNA expression.
Conclusions:
- Curcumin demonstrates a novel therapeutic mechanism for PSD via the GAS5/miR-10b/BDNF regulatory axis.
- This pathway involves GAS5 sponging miR-10b to modulate BDNF expression, offering a new target for PSD treatment.
