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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Updated: Dec 16, 2025

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders
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Multiscale Tomographic Analysis for Micron-Sized Particulate Samples.

Ralf Ditscherlein1, Orkun Furat2, Mathieu de Langlard2

  • 1Institute of Mechanical Process Engineering and Mineral Processing, Technische Universität Bergakademie Freiberg, D-09599Freiberg, Germany.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|July 7, 2020
PubMed
Summary
This summary is machine-generated.

Accurate 3D particle characterization is key for separation processes and material development. Combining micro-computed tomography (CT) and nano-CT effectively analyzes particle size and shape distributions across scales.

Keywords:
multidimensional particle characterizationmultiscale X-ray tomographyparametric copulastatistical image analysis

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Area of Science:

  • Materials Science
  • Chemical Engineering
  • Analytical Chemistry

Background:

  • Accurate three-dimensional (3D) characterization of micro- and nanometer-sized particles is crucial for optimizing separation processes.
  • Particle properties directly influence selectivity and yield, impacting functional material development.

Purpose of the Study:

  • To investigate the 3D characterization of mixed particle systems with varying aspect ratios and sizes.
  • To evaluate the effectiveness of computed tomographic (CT) measurements at different scales for particle analysis.

Main Methods:

  • Immobilization of mixed spherical glass particles and glass fibers in a wax matrix.
  • Acquisition of 3D data using X-ray micro-computed tomography (micro-CT) and nano-computed tomography (nano-CT) at different magnifications.
  • Modeling of particle property distributions using parametric copulas.

Main Results:

  • Micro-CT effectively characterized glass fiber dimensions (diameter and length) across the entire sample volume.
  • High-resolution nano-CT, combined with micro-CT, was necessary for accurate characterization of spherical particles.
  • Parametric copulas provided a statistically representative method for modeling multidimensional particle correlations.

Conclusions:

  • A multi-scale CT approach is essential for comprehensive 3D particle characterization, especially for systems with diverse particle morphologies.
  • Advanced statistical modeling, such as copulas, can capture complex correlations within particle systems.
  • This methodology enhances the understanding and optimization of separation processes and material design.