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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Forced Transdifferentiation01:28

Forced Transdifferentiation

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Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
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Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Updated: Dec 16, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

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Alternative splicing in mesenchymal stem cell differentiation.

Jung Woo Park1, Siyi Fu1, Borong Huang1

  • 1Center for Reproduction, Development, and Aging and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau, People's Republic of China.

Stem Cells (Dayton, Ohio)
|July 7, 2020
PubMed
Summary
This summary is machine-generated.

Alternative splicing (AS) plays a critical role in mesenchymal stem cell (MSC) differentiation into bone, cartilage, and fat cells. This review highlights key AS events and their regulatory mechanisms in MSCs.

Keywords:
ESC differentiationMSC differentiationRNA-binding proteinsadipogenicalternative splicingchondrogenicmesenchymal stem cellsneural differentiationosteogenic

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Stem Cell Research

Background:

  • Mesenchymal stem cell (MSC) differentiation is crucial for development and tissue homeostasis.
  • Dysregulation of MSC differentiation is implicated in diseases like obesity and osteoporosis.
  • Transcriptional regulation has been the primary focus for understanding MSC differentiation.

Purpose of the Study:

  • To review recent advancements in understanding the role of alternative splicing (AS) in MSC differentiation.
  • To highlight key AS events influencing osteocyte, chondrocyte, and adipocyte differentiation.
  • To discuss the regulatory mechanisms governing AS in MSCs.

Main Methods:

  • Literature review of recent studies on alternative splicing and MSC differentiation.
  • Cataloguing and analysis of key AS events.
  • Discussion of regulatory pathways involved in AS.

Main Results:

  • Alternative splicing is an emerging regulatory mechanism in MSC differentiation.
  • Specific AS events significantly modulate differentiation towards osteogenic, chondrogenic, and adipogenic lineages.
  • Understanding AS regulation provides new insights into MSC function.

Conclusions:

  • Alternative splicing is a critical, yet often overlooked, regulator of MSC differentiation.
  • Targeting AS pathways may offer novel therapeutic strategies for MSC-related diseases.
  • Further research into AS mechanisms is essential for advancing stem cell biology.