Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 16, 2025

Quantifying Mixing using Magnetic Resonance Imaging
07:33

Quantifying Mixing using Magnetic Resonance Imaging

Published on: January 25, 2012

11.3K

Component Quantification in Solids with the Mixture Analysis Using References Method.

Dirk Stueber1, Zachary E X Dance1

  • 1Department of Analytical Research and Development, Merck Research Laboratories, Merck & Company, Inc., Rahway, New Jersey 07065, United States.

Analytical Chemistry
|July 7, 2020
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Quantitative Analysis01:12

Quantitative Analysis

1.1K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.1K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.2K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.2K
Volatilization01:10

Volatilization

3.9K
Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...
3.9K
Qualitative Analysis01:10

Qualitative Analysis

1.1K
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
1.1K
Gravimetry: Overview01:05

Gravimetry: Overview

11.2K
Gravimetric analysis is a quantitative method where the analyte is isolated and weighed directly or after conversion into a substance of known composition. Gravimetric analysis can be classified as precipitation, electrogravimetry, volatilization, and particulate gravimetry, based on the method used to isolate the analyte.
In precipitation gravimetry, the analyte is converted into a precipitate and weighed. For example, the silver content in a sample can be estimated by precipitating and...
11.2K
Classifying Matter by Composition03:35

Classifying Matter by Composition

88.1K
Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated. 
A mixture is composed of two or...
88.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Injection Mass Spectrometry and iFishMass for the High-Throughput Analysis of Antibody Modifications.

ACS pharmacology & translational science·2026
Same author

Small-Angle X-ray Scattering as a Powerful Tool for Phase and Crystallinity Assessment of Monoclonal Antibody Crystallites in Support of Batch Crystallization.

Molecular pharmaceutics·2024
Same author

Engineering Hydroxylase Activity, Selectivity, and Stability for a Scalable Concise Synthesis of a Key Intermediate to Belzutifan.

Angewandte Chemie (International ed. in English)·2024
Same author

Diverse Catalytic Reactions for the Stereoselective Synthesis of Cyclic Dinucleotide MK-1454.

Journal of the American Chemical Society·2022
Same author

A kinase-cGAS cascade to synthesize a therapeutic STING activator.

Nature·2022

A new solid-state Nuclear Magnetic Resonance (NMR) method, Mixture Analysis using References (MAR), accurately quantifies components in solid mixtures. This efficient technique offers advantages over traditional methods for pharmaceutical ingredient analysis and beyond.

Area of Science:

  • Solid-state chemistry
  • Materials science
  • Analytical chemistry

Background:

  • Quantifying different physical forms of the same chemical compound in solid mixtures is challenging.
  • Active pharmaceutical ingredients (APIs) often exist as polymorphs, solvates, or hydrates, impacting stability and performance.
  • Accurate quantification of these forms is crucial for API development and quality control.

Purpose of the Study:

  • To introduce and evaluate a novel solid-state Nuclear Magnetic Resonance (NMR) based method for quantifying components in solid mixtures.
  • To assess the applicability and performance of the Mixture Analysis using References (MAR) method using various model systems.
  • To compare the efficiency and accuracy of MAR against traditional quantification techniques.

Main Methods:

More Related Videos

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy
03:49

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Published on: June 10, 2019

7.6K
Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

11.2K

Related Experiment Videos

Last Updated: Dec 16, 2025

Quantifying Mixing using Magnetic Resonance Imaging
07:33

Quantifying Mixing using Magnetic Resonance Imaging

Published on: January 25, 2012

11.3K
Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy
03:49

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Published on: June 10, 2019

7.6K
Quantifying X-Ray Fluorescence Data Using MAPS
14:58

Quantifying X-Ray Fluorescence Data Using MAPS

Published on: February 17, 2018

11.2K
  • Development of the Mixture Analysis using References (MAR) method.
  • Utilizing weighted pure component reference spectra in a linear combination fitting procedure.
  • Analysis of 13C and 19F cross-polarization magic-angle-spinning NMR data from model systems.

Main Results:

  • The MAR method accurately quantifies components in solid mixtures, achieving accuracy comparable to conventional methods.
  • MAR demonstrates significant efficiency advantages, requiring less experimental and computational time.
  • The method shows robustness and general applicability across various model systems of differing complexity.

Conclusions:

  • MAR provides a highly accurate and efficient alternative for quantifying components in solid mixtures.
  • The method's speed, robustness, and broad applicability make it valuable for pharmaceutical development.
  • The MAR protocol is readily adaptable to non-pharmaceutical molecular systems in diverse chemical fields.