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Updated: Dec 15, 2025
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Published on: April 27, 2018
Zebrafish brain RNA sequencing reveals that cell adhesion molecules are critical in brain aging
Begün Erbaba1, Özge Pelin Burhan1, Naz Şerifoğlu2
1Interdisciplinary Graduate Program in Neuroscience, Aysel Sabuncu Brain Research Center, Bilkent University, Ankara, Turkey; UNAM - National Nanotechnology Research Center and Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey; Department of Molecular Biology and Genetics Zebrafish Facility, Bilkent University, Ankara, Turkey.
Abstract:
Brain aging is a complex process, which involves multiple pathways including various components from cellular to molecular. This study aimed to investigate the gene expression changes in zebrafish brains through young-adult to adult, and adult to old age. RNA sequencing was performed on isolated neuronal cells from zebrafish brains. The cells were enriched in progenitor cell markers, which are known to diminish throughout the aging process. We found 176 statistically significant, differentially expressed genes among the groups, and identified a group of genes based on gene ontology descriptions, which were classified as cell adhesion molecules. The relevance of these genes was further tested in another set of zebrafish brains, human healthy, and Alzheimer's disease brain samples, as well as in Allen Brain Atlas data. We observed that the expression change of 2 genes, GJC2 and ALCAM, during the aging process was consistent in all experimental sets. Our findings provide a new set of markers for healthy brain aging and suggest new targets for therapeutic approaches to neurodegenerative diseases.
