Epitope Analysis of Murine and Chimeric Monoclonal Antibodies Recognizing the Cancer Testis Antigen PRAME
V A Misyurin1, Yu P Finashutina2, A A Turba3
1Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation, Moscow, Russia. vsevolod.misyurin@gmail.com.
Doklady. Biochemistry and Biophysics
|July 8, 2020
Abstract:
We investigated the epitope specificity of different monoclonal antibodies recognizing the cancer testis antigen PRAME. Antibody 5D3 binds to the fragment of PRAME corresponding to 160-180 amino acid residues. Antibodies 6H8 and F11 bind to the fragment corresponding to 180-200 amino acid residues of PRAME. These antibodies retained the ability to recognize these PRAME fragments after chimerization.


