Structural and Functional Diversification of SEPALLATA Genes TM5 and RIN in Tomato Species (Section Lycopersicon)
M A Slugina1, E A Dyachenko2, E Z Kochieva2,3
1Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia. mashinmail@mail.ru.
Abstract:
New TOMATO MADS 5 (TM5) homologous genes were identified in evolutionarily recent, red-fruited and more ancient, wild green-fruited tomato species. It was shown that the identified TM5 homologs belong to the SEPALLATA3 clade; thus, the SEP subfamily diversification was characterized. For the first time, the TM5 and RIN co-expression pattern was determined in flowers, immature green fruits, and ripe fruits of Solanum lycopersicum and in five wild tomato species. It was shown that, regardless of the species, the level of TM5 transcription in flowers was higher than that of RIN, whereas in fruits it was lower than the level of RIN transcription. The data obtained suggest that TM5, together with other transcription factors RIN and SlCMB1, is involved in the regulation of fruit development and ripening.
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