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Total Emission Time Resolved Decay: a Method for Measurement and Resolution of Broad-Band Emission
Suraj Kumar Panigrahi1, Ashok Kumar Mishra2
1Indian Institute of Technology, Madras, Chennai, 600036, India.
Journal of Fluorescence
|July 8, 2020
Summary
A new method, Total Emission Time Resolved Decay (TETRD), rapidly captures wavelength-dependent fluorescence decay. This technique simplifies analysis of complex lifetime distributions, offering advantages in experimental design and data acquisition for fluorescence spectroscopy.
Area of Science:
- Spectroscopy
- Photochemistry
- Materials Science
Background:
- Time-resolved fluorescence spectroscopy is crucial for understanding molecular dynamics.
- Conventional methods like time-resolved emission spectra (TRES) can be time-consuming.
- Analyzing complex fluorescence decay profiles requires robust analytical approaches.
Purpose of the Study:
- To introduce and validate a novel time-resolved fluorescence data acquisition technique called Total Emission Time Resolved Decay (TETRD).
- To demonstrate the utility of TETRD for analyzing systems with discrete and continuously distributed fluorescence lifetimes.
- To highlight the advantages of TETRD in terms of data acquisition speed and experimental flexibility.
Main Methods:
- TETRD data acquisition using zero-order diffraction of an emission grating in a time-correlated single-photon counting (TCSPC) instrument.
- Analysis of TETRD data using global analysis for discrete lifetime components.
- Application of stretched exponential fitting (StrEF) for continuous lifetime distribution systems.
Main Results:
- TETRD successfully captures comprehensive wavelength-dependent fluorescence decay information.
- Global analysis of TETRD data yields pre-exponentials and amplitudes that faithfully represent individual component emissions.
- StrEF analysis of TETRD data provides accurate average lifetimes and a heterogeneity factor (h) indicating local system heterogeneity.
- Lifetimes obtained via TETRD correlate well with those from conventional TRES measurements.
Conclusions:
- TETRD is an efficient technique for rapid acquisition of time-resolved fluorescence data.
- The method allows for convenient analysis of multi-component decay profiles using established methods like global analysis and StrEF.
- TETRD enhances experimental design by enabling the incorporation of additional variables, making it a versatile tool in fluorescence spectroscopy.
Keywords:
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