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Updated: Dec 15, 2025
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianum
Zeli Chen1,2,3, Rex Frimpong Anane4,5, Zhe Wang1,3
1Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.
Abstract:
The complete genome sequence of a putative novel potyvirus, tentatively named "Polygonatum kingianum virus 1" (PKgV1), infecting Polygonatum kingianum in China was determined (GenBank accession no. MK427056). PKgV1 has a genome organization that is typical of potyviruses, with a single large open reading frame (nt 123-9236) that encodes a 3037-aa polyprotein that is predicted to be cleaved into 10 mature proteins by virus-encoded proteases. Nine cleavage sites and several conserved motifs were identified in PKgV1 by comparative sequence analysis. Pairwise comparisons revealed that the PKgV1 polyprotein shares 52.0-56.2% nucleotide and 49.2-52.8% amino acid sequence identity with members of the genus Potyvirus. Phylogenetic analysis indicated that PKgV1 clustered with members of the genus Potyvirus and that it is closely related to but distinct from lettuce mosaic virus (LMV, accession no. KJ161186). These results suggest that Polygonatum kingianum virus 1 (PKgV1) is a new member of the genus Potyvirus of the family Potyviridae.
