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Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
Published on: June 17, 2015
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Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived
1Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Summary
The embryoid body test (EBT) accurately assesses developmental toxicity by measuring embryoid body size and cell viability. This validated method shows high accuracy, making it a reliable tool for identifying embryotoxicants efficiently.
Area of Science:
- Developmental toxicology
- Stem cell biology
- In vitro toxicology
Background:
- The embryoid body test (EBT) is a developmental toxicity assay.
- A prior pre-validation study demonstrated EBT's high accuracy (>80%) with 26 coded chemicals.
- Further validation is needed to assess inter-laboratory reproducibility and predictive capacity.
Purpose of the Study:
- To validate the embryoid body test (EBT) for developmental toxicity assessment.
- To evaluate the inter-laboratory reproducibility and predictive capacity of EBT.
- To update the EBT prediction model with accumulated data.
Main Methods:
- The second-phase validation study involved three laboratories testing coded chemicals.
- Inter-laboratory reproducibility was assessed using 5 common chemicals.
- Predictive capacity was evaluated using 10 chemicals per laboratory.
- An updated prediction model was used for analysis.
Main Results:
- Inter-laboratory reproducibility analysis yielded 87% accuracy, 78% sensitivity, and 100% specificity.
- Predictive capacity analysis showed 80% accuracy, 78% sensitivity, and 81% specificity.
- The updated prediction model improved accuracy with more data.
Conclusions:
- The embryoid body test (EBT) is a reliable method for classifying embryotoxicants.
- EBT offers accurate developmental toxicity testing with high sensitivity and specificity.
- The test is efficient, requiring minimal time and effort for developmental toxicity evaluation.
