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Standardizing luminescence nanothermometry for biomedical applications.

Artur Bednarkiewicz¹, Lukasz Marciniak¹, Luís D Carlos²

¹Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Poland. a.bednarkiewicz@intibs.pl l.marciniak@intibs.pl.

|July 8, 2020

Summary

Luminescence nanothermometry offers precise remote temperature sensing. This perspective proposes standardization guidelines to overcome reproducibility issues and ensure reliable nanothermometer use in biomedical applications.

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Area of Science:

Optics and Photonics
Materials Science
Biomedical Engineering

Background:

Luminescence nanothermometry is a rapidly advancing field for accurate, remote, all-optical thermal sensing.
Despite progress, significant challenges hinder reproducibility and reliable quantitative assessment of nanothermometers.
These challenges include a lack of universal evaluation rules and unclear understanding of thermal response mechanisms and environmental dependencies.

Purpose of the Study:

To critically discuss the obstacles impeding the reproducibility and quantitative assessment of luminescence nanothermometers.
To propose a series of standardization guidelines for the design, optimization, and application of these sensors.
To facilitate the scientific community's acceptance of luminescence thermometry as a reliable tool for remote temperature determination.

Main Methods:

Perspective article format, synthesizing existing knowledge and identifying key challenges.
Critical discourse on the limitations of current nanothermometry practices.
Formulation of proposed standardization guidelines for nanothermometer evaluation.

Main Results:

Identification of key issues hindering nanothermometer reliability: lack of universal evaluation rules, incorrect mechanistic assumptions, and external condition dependencies.
Proposal of specific standardization guidelines to address these identified problems.
Establishment of a framework for improved quantitative assessment and intentional design of nanothermometers.

Conclusions:

Addressing the identified challenges through standardization is crucial for advancing luminescence nanothermometry.
Standardized guidelines will enhance the reliability and reproducibility of nanothermometers.
This work is a foundational step towards the widespread adoption of luminescence thermometry in biomedical applications.