Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.2K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.2K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Wearable Motion Capture Dataset for Gait Analysis Using IMUs and Shank-Mounted Egocentric Cameras.

Scientific data·2026
Same author

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
Same author

Carcinogenicity prediction via multi-task learning of cross-organ representations with attention mechanisms.

Briefings in bioinformatics·2026
Same author

Omics-Guided Insights into Nanoparticle Complexity and Neural Regeneration.

Biosensors·2026
Same author

Integrating high-fidelity hiPSC-cardiomyocytes with AI-driven modeling for enhanced proarrhythmic risk assessment.

Archives of toxicology·2026
Same author

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026

Related Experiment Video

Updated: Dec 15, 2025

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
09:36

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

12.6K

Epitranscriptomic N6-Methyladenosine Modification Is Required for Direct Lineage Reprogramming into Neurons.

Hwan Choi1, Soonbong Baek2, Byounggook Cho1

  • 1Department of Biomedical Engineering, Dongguk University, Seoul 04620, South Korea.

ACS Chemical Biology
|July 8, 2020
PubMed
Summary

N6-methyladenosine (m6A) modification is crucial for direct lineage reprogramming into induced neuronal cells (iNs). Enhancing m6A levels boosts iN generation efficiency, highlighting epitranscriptomic remodeling

More Related Videos

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

8.1K
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

10.9K

Related Experiment Videos

Last Updated: Dec 15, 2025

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
09:36

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

Published on: May 12, 2014

12.6K
Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

8.1K
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

10.9K

Area of Science:

  • Epitranscriptomics
  • Cellular reprogramming
  • Neuroscience

Background:

  • N6-methyladenosine (m6A) is a key epitranscriptomic modification in eukaryotic mRNA.
  • m6A plays vital roles in diverse biological processes.
  • Understanding m6A's role in cell fate conversion is an emerging area of research.

Purpose of the Study:

  • To investigate the role of m6A modification in direct lineage reprogramming into induced neuronal cells (iNs).
  • To determine if m6A is required for the mRNA remodeling necessary for neuronal conversion.
  • To identify specific targets of m6A involved in iN generation.

Main Methods:

  • Utilized Mettl3 knockdown and overexpression to manipulate m6A methylation levels.
  • Assessed the efficiency of direct lineage reprogramming into iNs under varying m6A conditions.
  • Identified and analyzed the role of transcription factor Btg2 as an m6A target.

Main Results:

  • m6A modification is essential for the remodeling of specific mRNAs during neuronal direct conversion.
  • Inhibition of m6A methylation via Mettl3 knockdown reduced reprogramming efficiency.
  • Overexpression of Mettl3 enhanced the efficiency of iN generation.
  • Transcription factor Btg2 was identified as a functional target of m6A crucial for iN generation.

Conclusions:

  • Epitranscriptomic remodeling via m6A modification is critical for successful cell fate conversion into iNs.
  • m6A levels directly influence the efficiency of induced neuronal cell generation.
  • Targeting m6A pathways, including factors like Btg2, offers potential strategies for enhancing neuronal reprogramming.