Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

1.2K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.2K
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

1.3K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
1.3K
Microbial Fermentation01:23

Microbial Fermentation

1.1K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Profiling Active Low-Abundance Microbes in As/Sb-Contaminated Soils via d-Amino Acid-Based In Situ Labeling.

Environmental science & technology·2026
Same author

Function-Oriented and Waste-Derived Single-Atom Catalysts for Water Purification.

Chemical reviews·2026
Same author

Molecular-Level Perturbations of Dissolved Organic Matter Driven by Episodic Firecracker Residue Leaching.

Environmental science & technology·2026
Same author

Self-driven tandem alcoholysis for full upcycling of waste polycarbonate plastics.

Nature communications·2026
Same author

Structural and Electronic Features-Integrated Machine Learning Framework for High-Throughput Prediction of Organic Pollutant Reactivity.

Environmental science & technology·2026
Same author

Active Site Engineering of Mn-Doped CeO<sub>2</sub> Nanozymes: Enhanced Reactivity and Specificity for Colorimetric Detection of Perfluorooctanoic Acid in Waters.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Dec 15, 2025

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

2.1K

Probing Microbial Extracellular Respiration Ability Using Riboflavin.

Feng Zhang1,2, Jing-Hang Wu1, Han-Qing Yu1

  • 1CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Analytical Chemistry
|July 8, 2020
PubMed
Summary
This summary is machine-generated.

A new riboflavin-based fluorescence method accurately quantifies extracellular electron transfer (EET) in electrochemically active bacteria (EAB). This tool aids in identifying EAB and understanding their electron transfer mechanisms in various environments.

More Related Videos

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
06:17

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Published on: August 13, 2021

2.7K
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
09:39

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Published on: June 2, 2023

2.4K

Related Experiment Videos

Last Updated: Dec 15, 2025

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

2.1K
Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
06:17

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Published on: August 13, 2021

2.7K
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
09:39

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Published on: June 2, 2023

2.4K

Area of Science:

  • Microbiology
  • Electrochemistry
  • Biotechnology

Background:

  • Electrochemically active bacteria (EAB) are crucial for environmental, energy, and geoscientific applications due to their extracellular electron transfer (EET) capabilities.
  • Accurate and rapid quantification of EAB's EET ability remains a significant challenge, hindering their broader application and study.

Purpose of the Study:

  • To develop a facile, accurate, and in situ method for measuring the EET ability of EAB.
  • To quantify the single-cellular EET ability of prominent EAB species, Geobacter sulfurreducens DL-1 and Shewanella oneidensis MR-1.
  • To enable quantitative identification of EET-related c-type cytochromes in Shewanella oneidensis MR-1.

Main Methods:

  • Development of a novel riboflavin-based fluorescence assay.
  • In situ measurement of single-cellular EET currents.
  • Quantitative analysis of outer membrane c-type cytochromes.

Main Results:

  • The riboflavin-based fluorescence method successfully quantified the single-cellular EET ability of Geobacter sulfurreducens DL-1 (60.29 ± 13.02 fA) and Shewanella oneidensis MR-1 (2.11 ± 0.47 fA).
  • The method allowed for the quantitative identification of EET-related c-type cytochromes in Shewanella oneidensis MR-1.
  • Demonstrated the method's utility for rapid EAB identification and mechanism elucidation.

Conclusions:

  • The developed riboflavin-based fluorescence method offers a valuable tool for the rapid and accurate assessment of EAB EET ability.
  • This technique facilitates the identification of EAB in diverse environmental settings.
  • Provides insights into the electron transfer mechanisms and c-type cytochrome functions in EAB.