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PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer

Hong-Kun Wu1, Chang Liu1, Xin-Xing Li2

  • 1Department of Laboratory Medicine, Changzheng Hospital, Naval Medical University, Shanghai 200003, P.R. China.

Aging
|July 8, 2020
PubMed

Insights

Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) regulate PHLPP2 in colon cancer. LINC00402, LINC00461, and SFTA1P act as competing endogenous RNAs (ceRNAs) for PHLPP2, influencing tumor progression.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • The tumor suppressor role of PHLPP2 in colon cancer is established.
  • The involvement of long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) in regulating PHLPP2 in colon cancer remains to be fully elucidated.

Purpose of the Study:

  • To investigate the regulatory roles of lncRNAs and miRNAs on PHLPP2 in colon cancer.
  • To explore the potential of PHLPP2, lncRNAs, and miRNAs as therapeutic targets for colon cancer.

Main Methods:

  • Gene expression analysis using RT-qPCR and Western blot.
  • In vitro studies in HT-29 colon cancer cells.
  • In vivo studies using a mouse tumor xenograft model.

Main Results:

  • PHLPP2 was found to be downregulated in colon cancer and is a target of miR-141 and miR-424.
  • Upregulation of PHLPP2 and downregulation of miR-141/miR-424 suppressed colon cancer cell proliferation, migration, invasion, EMT, and promoted apoptosis.
  • LINC00402, LINC00461, and SFTA1P were identified as ceRNAs for PHLPP2 by competitively binding to miR-141 and miR-424, enhancing PHLPP2's suppressive effects.

Conclusions:

  • PHLPP2 exhibits suppressive effects in colon cancer pathogenesis.
  • LINC00402, LINC00461, and SFTA1P function as ceRNAs for PHLPP2 via interaction with miR-141 and miR-424, highlighting a novel regulatory network in colon cancer.
Keywords:
EMTPHLPP2ceRNAlncRNAmiRNA

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