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Prospective partition analysis of independently assorted sets. Accessory elements supporting clonal lymphoid
J Kettman1, K Burnham, I Lefkovits
1Basel Institute for Immunology, Switzerland.
Journal of Immunological Methods
|November 10, 1988
Summary
Accessory cells are crucial for T cell proliferation. A threshold of over 20 accessory cells is needed to promote T cell growth and achieve clonal kinetics in vitro.
Area of Science:
- Immunology
- Cell Biology
Background:
- Accessory cells are essential for mitogen-induced clonal proliferation of lymphocytes.
- T cell activation by concanavalin A is dependent on accessory cells.
Purpose of the Study:
- To investigate the role and kinetics of accessory cells in T cell proliferation.
- To determine the threshold of accessory cells required for T cell clonal growth.
Main Methods:
- In vitro culture of murine spleen cells to generate accessory cells.
- Partitioning of cultures to assess accessory cell distribution.
- Dispensing varying numbers of T cells into cultures with accessory cells.
Main Results:
- Accessory cells were found to be essential for T cell clonal proliferation.
- Neither accessory cells nor responding T cells showed single hit kinetics initially.
- A threshold of over 20 adherent accessory cells was identified as necessary for promoting T cell growth and achieving single hit kinetics.
Conclusions:
- Accessory cells play a critical role in supporting T cell clonal expansion.
- The number of accessory cells directly influences the kinetics of T cell proliferation.
- A specific density of accessory cells is required for predictable T cell clonal growth patterns.