Related Experiment Video
Updated: Dec 15, 2025
Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy
Published on: February 25, 2021
Simultaneous Pre-Concentration and HPLC-MS/MS Quantification of Phycotoxins and Cyanotoxins in Inland and Coastal
Francesca Merlo1, Federica Maraschi1, Davide Piparo1
1Department of Chemistry, University of Pavia, 27100 Pavia, Italy.
Abstract:
The purpose of this study was to set up a sensitive method for the simultaneous determination of phycotoxins and cyanotoxins-Emerging pollutants with different structures and harmful properties (hepatotoxicity, neurotoxicity and cytotoxicity)-In environmental waters. Due to the low concentrations detected in these samples, a pre-concentration step is required and here it was performed in a single step with a commercial cartridge (Strata™-X), achieving enrichment factors up to 200 and satisfactory recovery (R = 70-118%) in different aqueous matrices. After solid-phase extraction (SPE), toxins were separated and quantified by High Performance Liquid Chromatography- Heated ElectroSpray Ionisation Tandem Mass Spectrometry (HPLC-HESI-MS/MS) in Multiple Reaction Monitoring (MRM) mode. An analytical evaluation of the proposed method was done based on the analytical figures of merit, such as precision and trueness, linearity, selectivity, and sensitivity, and it turned out to be a robust tool for the quantification of ng L-1 levels, phycotoxins and cyanotoxins in both freshwater and saltwater samples.
More Related Videos
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
10:34Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
Published on: August 3, 2016