Related Experiment Video

Updated: Dec 15, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.4K

Autophagy Roles in Genome Maintenance

Susanna Ambrosio1, Barbara Majello2

  • 1Telethon Institute of Genetics and Medicine (TIGEM), 80078 Pozzuoli, Naples, Italy.

Cancers
|July 9, 2020
PubMed

Insights

Autophagy, a cellular process, is increasingly linked to maintaining genome integrity and responding to DNA damage. Understanding this connection is crucial for developing new cancer therapies targeting this pathway.

Area of Science:

  • Cellular Biology
  • Molecular Oncology
  • Genetics

Background:

  • A growing body of evidence highlights a significant correlation between autophagy and the maintenance of genome integrity.
  • Autophagy plays a role in preventing genomic instability and is involved in DNA damage response and cell fate determination.
  • The interplay between autophagy and DNA damage response is particularly relevant in the context of cancer, but remains incompletely understood.

Purpose of the Study:

  • To review the current understanding of the complex relationship between autophagy and genome integrity.
  • To discuss the implications of this crosstalk for cancer cell survival.
  • To explore the potential of targeting the autophagy pathway in cancer therapy.

Main Methods:

  • Literature review of recent research on autophagy and genome integrity.
  • Analysis of mechanisms linking autophagy to DNA damage response.
  • Discussion of experimental and clinical data regarding autophagy modulation in cancer.

Main Results:

  • Autophagy is implicated in multiple mechanisms that safeguard genome stability.
  • The cellular response to DNA damage involves autophagic processes.
  • Dysregulation of autophagy can impact cancer cell survival and treatment outcomes.

Conclusions:

  • The crosstalk between autophagy and genome integrity is a complex but critical area of cancer research.
  • Targeting autophagy presents a promising, yet challenging, therapeutic strategy for various malignancies.
  • Further investigation is needed to fully elucidate the consequences of modulating autophagy in cancer treatment.
Keywords:
DNA damage repairDNA damage responseautophagycancer therapy

More Related Videos

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

18.1K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

4.0K

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.5K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.6K
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
16.6K
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
199.9K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.8K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.9K