Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-cell adhesion mediated by CD8 and MHC class I molecules.

A M Norment1, R D Salter, P Parham

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143.

Nature
|November 3, 1988
PubMed
Summary

CD8 molecules directly bind to MHC class I molecules, mediating cell adhesion. This interaction occurs independently of the T-cell receptor, clarifying CD8

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Segmented filamentous bacteria take the stage.

Mucosal immunology·2010
Same author

Lymphoid tissue inducer cells in intestinal immunity.

Current topics in microbiology and immunology·2006
Same author

CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney.

Kidney international·2006
Same author

Immunogenetics of killer-cell immunoglobulin-like receptors.

Tissue antigens·2003
Same author

Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002.

Tissue antigens·2003
Same author

Human KIR sequences 2003.

Immunogenetics·2003

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Interactions

Background:

  • CD4 and CD8 are T-cell surface glycoproteins defining distinct T-cell subsets.
  • T cells expressing CD4 recognize antigens presented by MHC class II molecules.
  • T cells expressing CD8 recognize antigens presented by MHC class I molecules.

Purpose of the Study:

  • To investigate the direct binding interaction between CD8 molecules and MHC class I molecules.
  • To determine if CD8 can mediate cell adhesion with MHC class I in the absence of the T-cell receptor.

Main Methods:

  • Development of a cell-cell binding assay.
  • Utilized human B-cell lines expressing MHC class I molecules.
  • Co-cultured B cells with transfected cells expressing high levels of human CD8.

Related Experiment Videos

Main Results:

  • Demonstrated that CD8 and MHC class I molecules mediate cell-cell adhesion.
  • Confirmed direct binding between CD8 and MHC class I molecules.
  • This interaction occurs independently of the T-cell antigen receptor.

Conclusions:

  • CD8 directly binds to MHC class I molecules, facilitating cell adhesion.
  • This direct interaction plays a role in T-cell-target cell recognition.
  • Provides evidence for CD8's function beyond its association with the T-cell receptor.