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Updated: Sep 11, 2026

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Aseptic Laboratory Techniques: Plating Methods
Published on: May 11, 2012
Enumeration of Clostridium botulinum spores in meats by a pour-plate procedure
Canadian Journal of Microbiology
|June 1, 1977
Abstract:
Colonies of Clostridium botulinum could be easily distinguished from meat particles by supplementing Wynne agar with 0.4% egg yolk. The pour-plate method was suitable for enumeration of C. botulinum, provided the medium was covered with a layer of agar containing 0.01% dithiothreitol. Viable counts of heat-treated spores were consistently higher in Wynne agar supplemented with egg yolk (Wynne-EY agar) than in Wynne agar alone.

